96-well ultra-low attachment culture plate Search Results


u bottom low adherence 96 well plate  (Greiner Bio)
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Greiner Bio u bottom low adherence 96 well plate
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ultra low attachment 96 well plates  (Greiner Bio)
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Greiner Bio ultra low attachment 96 well plates
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ultralow attachment 96-well three-dimensional (3d) culture plates  (Corning Life Sciences)
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Corning Life Sciences ultralow attachment 96-well three-dimensional (3d) culture plates
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ultra-low attachment (ula) 96-well plates  (Nexcelom Bioscience)
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ultra-low attachment plates biofloattm 96-well plates  (faCellitate GmbH)
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ultra-low attachment 96-well round-bottomed plate with covalently bonded hydrogel that minimizes cell attachment ref. 7007  (Corning Life Sciences)
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96-well ultra-low binding u-shaped bottom culture plate corning #07-202-463  (Corning Life Sciences)
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ultra–low attachment round-bottom 96-well plate with fibronectin  (Corning Life Sciences)
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96-well ultra-low attachment culture plates corning tweksbury  (Corning Life Sciences)
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u-bottom ultra-low attachment 96-well plates  (Qing Dao Ama)
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sterile ultra-low attachment 96-well plate  (Corning Life Sciences)
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ultralow attachment (ula) 384-well plate  (Nexcelom Bioscience)
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Nexcelom Bioscience ultralow attachment (ula) 384-well plate
(A) The differentiation protocol of iPSCs into cerebral organoids. (B) Transfer of embryonic bodies (EBs) from an ultralow attachment (ULA) 384-well plate to a pillar plate as well as static and dynamic culture of cerebral organoids on the pillar plate. (C) Representative images of day 31 cerebral organoids cultured in a static condition using the 36PillarPlate/384DeepWellPlate and a dynamic condition using the 36PillarPlate/36PerfusionPlate. Scale bars: 350 μm. (D) The diameter of day 31 cerebral organoids cultured in static (SC) and dynamic (DC) conditions. n = 36 per condition. (E) ATP levels in day 31 cerebral organoids measured with CellTiter-Glo 3D cell viability assay kit. n = 18. (F) Neural biomarker expression in dynamically cultured cerebral organoids at days 0, 16, and 31, including OCT4 pluripotency marker, PAX6 forebrain neuroprogenitor marker, FOXG1 forebrain marker, TBR1 cortical preplate marker, MAP2 mature neuronal marker, TTR choroid plexus marker, CTIP2 deep cortical neuronal marker, and SOX2 proliferating neuroprogenitor marker. Statistical significance was assessed by one-way ANOVA. **** for p < 0.0001, *** for p < 0.001, ** for p < 0.01, * for p < 0.05, n. s. = not significant (p > 0.05). n = 10 – 12 per qPCR run. (G) Flow cytometry analysis of day 31 cerebral organoids cultured in a dynamic condition to assess the percentage of the cells expressing PAX6, FOXG1, TBR2, and CTIP2. n = 8.
Ultralow Attachment (Ula) 384 Well Plate, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


(A) The differentiation protocol of iPSCs into cerebral organoids. (B) Transfer of embryonic bodies (EBs) from an ultralow attachment (ULA) 384-well plate to a pillar plate as well as static and dynamic culture of cerebral organoids on the pillar plate. (C) Representative images of day 31 cerebral organoids cultured in a static condition using the 36PillarPlate/384DeepWellPlate and a dynamic condition using the 36PillarPlate/36PerfusionPlate. Scale bars: 350 μm. (D) The diameter of day 31 cerebral organoids cultured in static (SC) and dynamic (DC) conditions. n = 36 per condition. (E) ATP levels in day 31 cerebral organoids measured with CellTiter-Glo 3D cell viability assay kit. n = 18. (F) Neural biomarker expression in dynamically cultured cerebral organoids at days 0, 16, and 31, including OCT4 pluripotency marker, PAX6 forebrain neuroprogenitor marker, FOXG1 forebrain marker, TBR1 cortical preplate marker, MAP2 mature neuronal marker, TTR choroid plexus marker, CTIP2 deep cortical neuronal marker, and SOX2 proliferating neuroprogenitor marker. Statistical significance was assessed by one-way ANOVA. **** for p < 0.0001, *** for p < 0.001, ** for p < 0.01, * for p < 0.05, n. s. = not significant (p > 0.05). n = 10 – 12 per qPCR run. (G) Flow cytometry analysis of day 31 cerebral organoids cultured in a dynamic condition to assess the percentage of the cells expressing PAX6, FOXG1, TBR2, and CTIP2. n = 8.

Journal: bioRxiv

Article Title: Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity

doi: 10.1101/2024.03.11.584506

Figure Lengend Snippet: (A) The differentiation protocol of iPSCs into cerebral organoids. (B) Transfer of embryonic bodies (EBs) from an ultralow attachment (ULA) 384-well plate to a pillar plate as well as static and dynamic culture of cerebral organoids on the pillar plate. (C) Representative images of day 31 cerebral organoids cultured in a static condition using the 36PillarPlate/384DeepWellPlate and a dynamic condition using the 36PillarPlate/36PerfusionPlate. Scale bars: 350 μm. (D) The diameter of day 31 cerebral organoids cultured in static (SC) and dynamic (DC) conditions. n = 36 per condition. (E) ATP levels in day 31 cerebral organoids measured with CellTiter-Glo 3D cell viability assay kit. n = 18. (F) Neural biomarker expression in dynamically cultured cerebral organoids at days 0, 16, and 31, including OCT4 pluripotency marker, PAX6 forebrain neuroprogenitor marker, FOXG1 forebrain marker, TBR1 cortical preplate marker, MAP2 mature neuronal marker, TTR choroid plexus marker, CTIP2 deep cortical neuronal marker, and SOX2 proliferating neuroprogenitor marker. Statistical significance was assessed by one-way ANOVA. **** for p < 0.0001, *** for p < 0.001, ** for p < 0.01, * for p < 0.05, n. s. = not significant (p > 0.05). n = 10 – 12 per qPCR run. (G) Flow cytometry analysis of day 31 cerebral organoids cultured in a dynamic condition to assess the percentage of the cells expressing PAX6, FOXG1, TBR2, and CTIP2. n = 8.

Article Snippet: The collected cells were further dissociated into single cells through pipetting and seeded in an ultralow attachment (ULA) 384-well plate (ULA-384U-020, Nexcelom) at a seeding density of 1000 cells in each well containing 50 µL of the hESC medium.

Techniques: Cell Culture, Viability Assay, Biomarker Assay, Expressing, Marker, Flow Cytometry